幼年型喉乳头状瘤病裸鼠移植瘤模型的建立

doi:10.3969/j.issn.1674-5671.2012.02.20

中国癌症防治杂志 ›› 2012, Vol. 4 ›› Issue (2): 179-183.doi: 10.3969/j.issn.1674-5671.2012.02.20

幼年型喉乳头状瘤病裸鼠移植瘤模型的建立

广西医科大学研究生学院；广西医科大学第一附属医院耳鼻咽喉科

出版日期:2012-06-25 发布日期:2012-07-03
通讯作者: 温文胜 E-mail:wenwensheng2000@126.com

Establishment of a juvenile-onset recurrent respiratory papillomatosis（JO-RRP）transplanted tumor model in nude mice

Online:2012-06-25 Published:2012-07-03

摘要/Abstract

摘要： Objective To establish a juvenile-onset recurrent respiratory papillomatosis（JO-RRP）transplanted tumor model in nude mice. Methods Fresh tissues of laryngeal papilloma were obtained from 36 juvenile patients during operations.Tissues were cut into small pieces of approximately 1 mm3，transplanted into BALB/c nude mice subcutaneously，and then observed periodically. Results Tumors were successfully transplanted in 20 mice，corresponding to a tumor formation rate of 56%.The transplanted tumors presented histological characteristics similar to those of the tumors in humans.Virus HPV6/11 was confirmed in the transplanted tumor tissues by in situ hybridization and PCR. Conclusion A transplanted tumor model of JO-RRP has been established，which should provide a basis for studying juvenile laryngeal papillomatosis.

关键词: Laryngeal papilloma, Virus, Animal model

Abstract: Objective To establish a juvenile-onset recurrent respiratory papillomatosis（JO-RRP）transplanted tumor model in nude mice. Methods Fresh tissues of laryngeal papilloma were obtained from 36 juvenile patients during operations.Tissues were cut into small pieces of approximately 1 mm3，transplanted into BALB/c nude mice subcutaneously，and then observed periodically. Results Tumors were successfully transplanted in 20 mice，corresponding to a tumor formation rate of 56%.The transplanted tumors presented histological characteristics similar to those of the tumors in humans.Virus HPV6/11 was confirmed in the transplanted tumor tissues by in situ hybridization and PCR. Conclusion A transplanted tumor model of JO-RRP has been established，which should provide a basis for studying juvenile laryngeal papillomatosis.

Key words: Laryngeal papilloma, Virus, Animal model

韩琳, 唐朝贤, 李锦明, 吴森勇, 谢莹, 温文胜. 幼年型喉乳头状瘤病裸鼠移植瘤模型的建立[J]. 中国癌症防治杂志, 2012, 4(2): 179-183.

HAN Lin, TANG Chao-Xian, LI Jin-Ming, WU Sen-Yong, XIE Ying, WEN Wen-Sheng. Establishment of a juvenile-onset recurrent respiratory papillomatosis（JO-RRP）transplanted tumor model in nude mice[J]. Chinese Journal of Oncology Prevention and Treatment, 2012, 4(2): 179-183.